|“||Gobby, Meet the Web-Warriors!||”|
When Green Goblin traveled the multiverse to steal the DNA of the various Spider-men. Spider-Man was forced to follow him to stop him. After failing to prevent Green Goblin from collecting the DNA, Spider-Man recruited Green Goblin's victims to stop him.
It was briefly reformed during Contest of Champions, to evacuate other civilians, heroes and villains of Earth from the Grandmaster's imprisonment.
Spider-Man later reformed the team after Doctor Octopus teamed up with HYDRA and captured the Tricarrier transforming it into Hydra Island. Miles later traveled to Peter's world to stop his world's Goblin, but the Siege Perilous was destroyed and now he's stranded. He rejoined the Web-Warriors eventually taking the name Kid Arachnid to avoid the confusion of having two Spider-Mans.
Scarlet Spider was a spy for Doctor_Octopus all along. However, due to respect for Spider-Man's aunt, Aunt May, he realize when Spider-Man ask if Doc Ock cared for Scarlet, and Ock replies Scarlet is only a tool until he may somehow useless soon enough, thus changes his mind, but sacrifices his life to save the city.
Ever since the Siege Perilous was destroyed by Spider-Man on making the alternate Miles and Goblin stranded in the main universe, it shattered to across dimensions, leaving an universal phenomenal on causing every spider people, along with some of their relatives and their allies somehow moved to another universe for temporarily. It was then, Iron Fist sends Spider-Man and Kid Arachnid to K'un Lun to meet up Nick Fury, Doctor Strange, and Madame Web. This mission becomes a great opportunity for Kid Arachnid to return to his home universe, but forgot that he must bring his world's Goblin back as well. The true mastermind is Wolf Spider, an evil spider-person from another universe who attempt to use Siege Perilous to take over all realities under his knees. It is up to Spider-Man and Kid Arachnid to gather the remaining good spider-people, old and new allies alike to stop Wolf Spider. However in the end, after Wolf Spider is defeated, Miles soon decided to stay in the mainstream universe along with with his mother Rio Morales, leaving his universe under protection of Spider-Gwen in his place, to keep the connection of the Web Warriors members in touch between the realities.
With Scarlet Spider revealed to have survive the crash and seeks to find his true origin, Scarlet is permitted to return to Web Warriors, but not without recruiting his fellow Spider Slayers, who are in Arnim Zola, and formerly Doctor Octopus' control. In the same time upon Spider-Man and Mary Jane are found Scarlet Spider alive, Mary Jane becomes Spider-Woman due to being a perfect host for the Carnage symbiote since the events of The Symbiote Saga, with the help of Dr. Connors to completely control the symbiote, making her temporarily the latest addition to the Web Warriors. However, Kaine, an evil unstable Doc Ock and HYDRA created has more absolute powers to control the Slayers than Scarlet, leaving Scarlet is the only synthezoid Spider-Slayer to survive after the Web-Warriors manage to overload an Ultimate Spider-Slayer Kaine (a form after merging with Scarlet's former Spider-Slayer team).
- Spider-Man/Peter Parker (Leader)
- Agent Venom/Eugene Thompson
- Iron Spider/Amadeus Cho
- Scarlet Spider/Benjamin Reilly
- Ultimate Spider-Man/Kid Arachnid/Miles Morales (Originally from alternate reality)
- Spider-Woman/Mary Jane Watson (Temporarily, joined S.H.I.E.L.D. Academy)
- Spider-Man 2099/Miguel O' Hara
- Spider-Girl/Petra Parker
- Spider-Man Noir/Peter Parker
- Spider-Ham/Peter Porker
- Spyder-Knight/Peter Parker
- Blood Spider/Peter Parker
- Web Beard/Peter Parker
- Web-Slinger/Peter Parker
- Spider-Gwen/Gwendolyn Stacy
- Spider-Punk/Hobart Brown
- Spider-Man/Peter Parker '67
- Other Alternate Reality's Good Spider-Men
- Spider-Man's Classic Team
- New Warriors
- Spider Slayers (Synthezoids) (Defected)
- Sinister Six
- Lizard/Dr. Curt Connors
- Beetle/Abner Jenkins
- Batroc the Leaper/Georges Batroc
- Grizzly/Max Markham
- Shriek/Frances Barrison
- Shocker/Herman Schultz
- Grandmaster/En Dwi Gast
- Spider Slayers
- Some of them were told by Spider-Man that he is them while a few weren't.
- After the events with Wolf Spider, it seems they all know he is their counterpart.